Sometimes you just have to improvise in the lab...
ReadToday in the lab I was breaking up bacteria to extract a gene I inserted into them...
ReadToday in the lab I was mounting muscle biopsies on microscope slides after immunostaining them...
ReadToday in the lab I was doing an experiment involving brain cells kept in cell culture flasks...
ReadToday in the lab I was preparing a stock of a protein for long-term storage...
ReadToday in the lab I was breaking up bacteria to extract a gene I inserted into them...
ReadToday in the lab I was using a virus to transfer a specific gene into a mammalian cell...
ReadToday in the lab I was running a lot of quantitative PCR (qPCR) plates...
ReadToday in the lab I was using liquid nitrogen to snap-freeze brain tissue...
ReadToday in the lab I was using a multichannel pipette to speed up a biochemical assay...
ReadToday's experiment was a biochemical assay to determine the concentration of a protein released by brain cells...
ReadToday in the lab I was doing genotyping. Samples of DNA were collected from the latest litters of the lab's colonies and their genotype had to be determined to check which of them carry genetic mutations in specific genes...
ReadToday in the lab I was doing some genotyping to determine which DNA samples contained a specific mutation...
ReadToday in the lab I spent a long time dissecting some brains. During the dissection, I need to remove the meninges before I can proceed to collect the parts of the brain that are used for glial cell culture...
ReadToday in the lab I spent a long time cryopreserving some brain cells for later use. It took me approximately 3 hours without breaks to prepare only 10 samples...
ReadToday in the lab I was doing a miniprep experiment. This experiment allows the purification of plasmid DNA from bacterial cultures...
ReadToday in the lab I was dissecting neonatal brains, in order to get glial cells and culture them for my experiments...
ReadToday in the lab I was looking at brain microglia which were isolated from a mixed culture of both microglia and astrocytes three days earlier...
ReadOne of the things I did today in the lab, was to check the health of some cells growing in culture, and to estimate their numbers because they need to be of a certain confluency before I can use them for any experiments...
ReadToday in the lab I did a bacterial transformation experiment, then left the bacteria to grow on agar plates overnight, so that they would clone themselves and produce multiple copies of a gene I am interested in...
ReadToday in the lab, I inoculated four flasks of bacterial cultures, which will be left overnight for the bacterial to clone themselves and produce multiple copies of a piece of DNA that I put into them...
ReadToday in the lab I was using the confocal microscope to look at different cell types on brain slices...
ReadToday in the lab, I was analysing the calcium activity of the white matter in the corpus callosum (the area of the brain that connects the two hemispheres)...
ReadToday in the lab I was using the confocal microscope to look at different cell types in culture...
ReadToday in the lab, I spent a lot of my time on the computer, analysing the electrical activity of neurons that I had previously recorded during my electrophysiology experiments...
ReadToday in the lab I was doing an electrophysiology experiment, specifically known as the patch-clamp technique...
ReadToday in the lab I was analysing H&E stained brain slices to identify histopathological changes following a stroke...
ReadToday's experiment in the lab was all about staining brain slices with Hematoxylin & Eosin (H&E) for microscopy. In the timelapse below, you can see me performing the experiment, which lasted about 3.5 hours...
ReadToday in the lab, I spent the entire time doing image analysis, using the MRI scans collected during an experiment that was testing possible new treatments for stroke...
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